This Technical Report gives criteria for single laboratory validated methods of analysis for the determination of mycotoxins. The criteria and topics covered are accuracy, trueness, recovery, precision, measurement uncertainty, selectivity, applicability, linearity, limit of detection, limit of quantification, sensitivity, ruggedness, specificity. This report also contains information on terms and definitions, validation, standardization procedures and interlaboratory studies by international organizations (e.g. AOAC, CEN, ISO, IUPAC, IDF). Confirmatory methods and screening methods are described. The validation criteria specified for mycotoxins in general are given.